IGF1 Protein

Catalog No. ABIN7855699

This Recombinant IGF1 protein is expressed in CHO Cells.

Quick Overview for IGF1 Protein (ABIN7855699)

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: CHO Cells

Application: Cell Culture (CC)

Purity: ≥ 90.0% by SDS-PAGE

Grade: Animal-Free

Product Details

Purpose: rIGF-1 LR3 - Recombinant human Insulin-like growth factor-1 LR3

Characteristics: This product arrives as White lyophilized powder 

Endotoxin Level: Less than 5 EU/mg

Biological Activity Comment: The bioactivity determined by a cell proliferation assay using Balb/c 3T3 cell, corresponding to a specific activity of ≥ 1x10^6 Units/mg.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recommend to reconstitute rhIGF-1 LR3 at 100-200μg/ml concentration in sterile water. Further dilutions can be made in other aqueous buffer.

Buffer: It is lyophilized with mannitol as the stabilizer.

Handling Advice: Use as soon as possible after opening. Avoid freeze-thaw cycles

Storage: -20 °C

Storage Comment: Stored at temperatures -20°C for at least 36 months. Further dilutions can be made in other aqueous buffer. Use as soon as possible after opening. Please avoid freeze-thaw cycles.

Expiry Date: 36 months

Target Details

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Alternative Name: Insulin-like growth factor-1

Background: Human Insulin-like growth factor-1 (hIGF-1) is a hormone similar in molecular structure to insulin. It primarily functions through binding to its specific receptor, the insulin-like growth factor 1 receptor (IGF1R). IGF-1 is one of the most potent natural activators of the AKT signaling pathway, which stimulates cell growth and proliferation, and inhibits cell apoptosis. rhIGF-1 LR3 (rhIGF-1 Long Arg3) is an 83 amino acid analog of IGF-1 comprising the complete human IGF-1 sequence with the substitution of an Arg for the Glu at position 3 (hence R3), and a 13 amino acid extension peptide at the N-terminus. The rhIGF-1 LR3, purified by chromatography from E.coli, then lyophilized with Mannitol, has much higher stability and biological activity. The manufacture capability at hundreds of grams scales can meet your large bioprocessing needs.

Molecular Weight: 9.1kD

Pathways: RTK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation