MGMT Protein (His tag)
Quick Overview for MGMT Protein (His tag) (ABIN7856849)
Target
See all MGMT ProteinsProtein Type
Origin
Source
Application
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Purification tag / Conjugate
- This MGMT protein is labelled with His tag.
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Purpose
- Recombinant MGMT Protein
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Sequence
- Full Length
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Characteristics
- Recombinant full length human MGMT was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.
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Sterility
- Sterile
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O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) protein (His tag)
custom-made MGMT Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) protein (GST tag)MGMT Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) protein (His-IF2DI Tag)MGMT Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
O6-Methylguanine-DNA-Methyltransferase (MGMT) protein (Myc-DYKDDDDK Tag)MGMT Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Supplied as sterile 50 mM sodium phosphate, pH 7.5, 300 mM NaCl, 50-200 mM imidazole, 0-0.1 mM PMSF, 0.2-1.0 mM DTT, 10-25 % glycerol
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Preservative
- Dithiothreitol (DTT), PMSF
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Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -80 °C
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Storage Comment
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Product shipped on dry ice.
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Expiry Date
- 12 months
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- MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))
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Alternative Name
- MGMT
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Molecular Weight
- ~25 kDa
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Pathways
- DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus
Target
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