CD14 Protein (CD14) (AA 20-367)
Quick Overview for CD14 Protein (CD14) (AA 20-367) (ABIN7866003)
Target
See all CD14 ProteinsProtein Type
Biological Activity
Origin
Source
Purity
-
-
Protein Characteristics
- AA 20-367
-
Purpose
- Human CD14 Protein Recombinant
-
Sequence
- TTPEPCELDD EDFRCVCNFS EPQPDWSEAF QCVSAVEVEI HAGGLNLEPF LKRVDADADP RQYADTVKAL RVRRLTVGAA QVPAQLLVGA LRVLAYSRLK ELTLEDLKIT GTMPPLPLEA TGLALSSLRL RNVSWATGRS WLAELQQWLK PGLKVLSIAQ AHSPAFSCEQ VRAFPALTSL DLSDNPGLGE RGLMAALCPH KFPAIQNLAL RNTGMETPTG VCAALAAAGV QPHSLDLSHN SLRATVNPSA PRCMWSSALN SLNLSFAGLE QVPKGLPAKL RVLDLSCNRL NRAPQPDELP EVDNLTLDGN PFLVPGTALP HEGSMNSGVV P
-
Characteristics
-
Protein Size: Recombinant CD14 migrates as an approximately 36 kDa protein due to glycosylation under reducing conditions in SDS-PAGE.
Sequence Position: Optimized DNA sequence encoding Human CD14 mature chain was expressed in CHO. Recombinant Human Monocyte differentiation antigen CD14 encodes amino acid residues 20-367.
-
Endotoxin Level
- Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).
-
Biological Activity Comment
- Determined by the dose dependent activation of NF-κB in a RAW264 cell line
-
-
Want other Options for this Protein ?
!Discover Our Predefined Custom Proteins and Custom Protein Services!Your project requires further customization? Contact us and discover our custom protein solutions
-
CD14 (CD14) (AA 1-375) protein (GST tag)
CD14 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
CD14 (CD14) (AA 16-345) (Active) protein (His tag)CD14 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD14 (CD14) (AA 20-352) (Active) protein (His tag)CD14 Origin: Human Host: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. SDS, WB, Imm, ELISA, AcA Active
CD14 (CD14) (AA 18-345) (Active) protein (His-Fc tag)CD14 Origin: Mouse Host: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. SDS, WB, Imm, ELISA, AcA Active
CD14 (CD14) protein (His tag,Fc Tag)CD14 Origin: Mouse Host: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE
CD14 (CD14) protein (His tag)CD14 Origin: Rat Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
CD14 (CD14) protein (Fc Tag)CD14 Origin: Rat Host: HEK-293 Cells Recombinant > 93 % as determined by SDS-PAGE
CD14 (CD14) (AA 20-352) protein (His tag)CD14 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. ScA, IA, ADA, AM
CD14 (CD14) (AA 20-344) protein (His tag)CD14 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. ScA, IA, ADA, AM
CD14 (CD14) (AA 20-171) protein (His-IF2DI Tag)CD14 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
-
-
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Reconstitution
- A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
-
Buffer
- CD14 was Lyophilized from PBS, pH 7.2.
-
Storage
- 4 °C,-20 °C
-
Storage Comment
- The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Upon reconstitution, this product can be stored in working aliquots at 2°- 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
-
Expiry Date
- 24 months
-
-
- CD14
-
Alternative Name
- CD14
-
Molecular Weight
- 36 kDa
-
Pathways
- TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
Target
-
