DLL1 Protein (AA 18-723)

Catalog No. ABIN7866014

Recombinant DLL1 protein expressed in CHO Cells.

Quick Overview for DLL1 Protein (AA 18-723) (ABIN7866014)

Target: DLL1 (delta-Like 1 (DLL1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: CHO Cells

Purity: >95 % , as determined by SDS-PAGE and HPLC

Product Details

Protein Characteristics: AA 18-723

Purpose: Human DLL1 Protein Recombinant

Sequence: SGVFELKLQE FVNKKGLLGN RNCCRGGAGP PPCACRTFFR VCLKHYQASV SPEPPCTYGS AVTPVLGVDS FSLPDGGGAD SAFSNPIRFP FGFTWPGTFS LIIEALHTDS PDDLATENPE RLISRLATQR HLTVGEEWSQ DLHSSGRTDL KYSYRFVCDE HYYGEGCSVF CRPRDDAFGH FTCGERGEKV CNPGWKGPYC TEPICLPGCD EQHGFCDKPG ECKCRVGWQG RYCDECIRYP GCLHGTCQQP WQCNCQEGWG GLFCNQDLNY CTHHKPCKNG ATCTNTGQGS YTCSCRPGYT GATCELGIDE CDPSPCKNGG SCTDLENSYS CTCPPGFYGK ICELSAMTCA DGPCFNGGRC SDSPDGGYSC RCPVGYSGFN CEKKIDYCSS SPCSNGAKCV DLGDAYLCRC QAGFSGRHCD DNVDDCASSP CANGGTCRDG VNDFSCTCPP GYTGRNCSAP VSRCEHAPCH NGATCHERGH RYVCECARGY GGPNCQFLLP ELPPGPAVVD LTEKLEGQGG PF

Characteristics:

Protein Size: Recombinant DLL1 migrates as an approximately 57 kDa protein due to glycosylation under reducing conditions in SDS-PAGE.

Sequence Position: Optimized DNA sequence encoding Human DLL1 mature chain including a C-terminal 6xHis tag was expressed in CHO. Recombinant Human Delta-like protein 1 encodes amino acid residues 18-723.

Endotoxin Level: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).

Biological Activity Comment: DLL-1 inhibits the proliferation in THP-1 cells using a concentration of 3.0-5.0 μg/ml

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Buffer: DLL1 was Lyophilized from PBS, pH 7.2.

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Upon reconstitution, this product can be stored in working aliquots at 2°- 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Expiry Date: 24 months

Target Details

Target: DLL1 (delta-Like 1 (DLL1))

Alternative Name: DLL1

Molecular Weight: 57 kDa

Pathways: Notch Signaling, Stem Cell Maintenance