FGF18 Protein (AA 28-207)
Quick Overview for FGF18 Protein (AA 28-207) (ABIN7866020)
Target
See all FGF18 ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 28-207
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Purpose
- Human FGF18 Protein Recombinant
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Sequence
- AEENVDFRI HVENQTRARD DVSRKQLRLY QLYSRTSGKH IQVLGRRISA RGEDGDKYAQ LLVETDTFGS QVRIKGKETE FYLCMNRKGK LVGKPDGTSK ECVFIEKVLE NNYTALMSAK YSGWYVGFTK KGRPRKGPKT RENQQDVHFM KRYPKGQPEL QKPFKYTTVT KRSR
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Characteristics
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Protein Size: Recombinant FGF18 migrates as an approximately 19 kDa protein under reducing conditions in SDS-PAGE.
Sequence Position: Optimized DNA sequence encoding Human FGF18 mature chain including a C-terminal 6xHis tag was expressed in E. Coli. Recombinant Human Fibroblast growth factor 18 encodes amino acid residues 28-207.
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Endotoxin Level
- Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).
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Biological Activity Comment
- Determined by thymidine uptake by BaF3 cells expressing FGF-receptors is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2 x 10^6 units/mg.
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Fibroblast Growth Factor 18 (FGF18) (Active) protein
FGF18 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Fibroblast Growth Factor 18 (FGF18) (AA 29-207) protein (GST tag)FGF18 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Fibroblast Growth Factor 18 (FGF18) (AA 28-207) protein (His-IF2DI Tag)FGF18 Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
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Buffer
- FGF18 was Lyophilized from PBS, pH 7.2.
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Storage
- 4 °C,-20 °C
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Storage Comment
- The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Upon reconstitution, this product can be stored in working aliquots at 2°- 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
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Expiry Date
- 24 months
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- FGF18 (Fibroblast Growth Factor 18 (FGF18))
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Alternative Name
- FGF18
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Molecular Weight
- 19 kDa
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Pathways
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Target
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