FGF19 Protein (AA 25-216)
Quick Overview for FGF19 Protein (AA 25-216) (ABIN7866021)
Target
See all FGF19 ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 25-216
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Purpose
- Human FGF19 Protein Recombinant
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Sequence
- MRPLAFSDAG PHVHYGWGDP IRLRHLYTSG PHGLSSCFLR IRADGVVDCA RGQSAHSLLE IKAVALRTVA IKGVHSVRYL CMGADGKMQG LLQYSEEDCA FEEEIRPDGY NVYRSEKHRL PVSLSSAKQR QLYKNRGFLP LSHFLPMLPM VPEEPEDLRG HLESDMFSSP LETDSMDPFG LVTGLEAVRS PSFEK
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Characteristics
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Protein Size: Recombinant FGF19 migrates as an approximately 21 kDa protein under reducing conditions in SDS-PAGE.
Sequence Position: Optimized DNA sequence encoding Human FGF19 mature chain including a C-terminal 6xHis tag was expressed in E. Coli. Recombinant Human Fibroblast growth factor 19 encodes amino acid residues 25-216.
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Endotoxin Level
- Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).
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Biological Activity Comment
- Determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 for this effect is 15-25 μg/ml.
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Fibroblast Growth Factor 19 (FGF19) (AA 1-216) protein (GST tag)
FGF19 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Fibroblast Growth Factor 19 (FGF19) (AA 25-216) protein (His tag)FGF19 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Fibroblast Growth Factor 19 (FGF19) protein (His tag)FGF19 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by reducing SDS-PAGE. AM, ADA, IA, ScA
Fibroblast Growth Factor 19 (FGF19) (AA 23-216) protein (His-IF2DI Tag)FGF19 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
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Buffer
- FGF19 was Lyophilized from PBS, pH 7.2.
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Storage
- 4 °C,-20 °C
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Storage Comment
- The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Upon reconstitution, this product can be stored in working aliquots at 2°- 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
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Expiry Date
- 24 months
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- FGF19 (Fibroblast Growth Factor 19 (FGF19))
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Alternative Name
- FGF19
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Molecular Weight
- 21 kDa
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Pathways
- RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Target
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