NOV Protein (AA 32-357)

Catalog No. ABIN7866040

This Recombinant NOV protein is expressed in Escherichia coli (E. coli).

Quick Overview for NOV Protein (AA 32-357) (ABIN7866040)

Target: NOV (Nephroblastoma Overexpressed (NOV))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: >95 % , as determined by SDS-PAGE and HPLC

Product Details

Protein Characteristics: AA 32-357

Purpose: Human Nov Protein Recombinant

Sequence: MQVAATQRCP PQCPGRCPAT PPTCAPGVRA VLDGCSCCLV CARQRGESCS DLEPCDESSG LYCDRSADPS NQTGICTAVE GDNCVFDGVI YRSGEKFQPS CKFQCTCRDG QIGCVPRCQL DVLLPEPNCP APRKVEVPGE CCEKWICGPD EEDSLGGLTL AAYRPEATLG VEVSDSSVNC IEQTTEWTAC SKSCGMGFST RVTNRNRQCE MLKQTRLCMV RPCEQEPEQP TDKKGKKCLR TKKSLKAIHL QFKNCTSLHT YKPRFCGVCS DGRCCTPHNT KTIQAEFQCS PGQIVKKPVM VIGTCTCHTN CPKNNEAFLQ ELELKTTRGK M

Characteristics:

Protein Size: Recombinant Nov migrates as an approximately 36 kDa protein under reducing conditions in SDS-PAGE.

Sequence Position: Optimized DNA sequence encoding Human Nov mature chain was expressed in E. Coli. Recombinant Human Protein NOV homolog encodes amino acid residues 32-357.

Endotoxin Level: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).

Biological Activity Comment: Determined by a cell proliferation assay using BALB/c 3T3 cells. The expected ED50 for this effect is 1.0-2.0 μg/ml

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Buffer: Nov was Lyophilized from PBS, pH 7.2.

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Upon reconstitution, this product can be stored in working aliquots at 2°- 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Expiry Date: 24 months

Target Details

Target: NOV (Nephroblastoma Overexpressed (NOV))

Alternative Name: Nov

Molecular Weight: 36 kDa

Pathways: Smooth Muscle Cell Migration, Growth Factor Binding