NOV Protein (AA 1-357) (GST tag)
(2 references)-
- Target See all NOV Proteins
- NOV (Nephroblastoma Overexpressed (NOV))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-357
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This NOV protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
- Purpose
- NOV (Human) Recombinant Protein (P01)
- Sequence
- MQSVQSTSFC LRKQCLCLTF LLLHLLGQVA ATQRCPPQCP GRCPATPPTC APGVRAVLDG CSCCLVCARQ RGESCSDLEP CDEGSGLYCD RSADPSKQTG ICTAVEGDNC VFDGVIYRSG EKFQPSCKFQ CTCRDGQIGC VPRCQLDVLL PEPNCPAPRK VEVPGECCEK WICGPDEEDS LGGLTLAAYR PEATLGVEVS DSSVNCIEQT TEWTACSKSC GMGFSTRVTN RNRQCEMLKQ TRLCMVRPCE QEPEQPTDKK GKKCLRTKKS LKAIHLQFKN CTSLHTYKPR FCGVCSDGRC CTPHNTKTIQ AEFQCSPGQI VKKPVMVIGT CTCHTNCPKN NEAFLQELEL KTTRGKM
- Characteristics
- Human NOV full-length ORF ( AAH15028, 1 a.a. - 357 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Nephroblastoma Overexpressed (NOV) protein (His tag)
NOV Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
Nephroblastoma Overexpressed (NOV) (AA 28-357) protein (His tag)NOV Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Nephroblastoma Overexpressed (NOV) (AA 32-131) protein (GST tag)NOV Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Nephroblastoma Overexpressed (NOV) (AA 32-357) protein (His tag)Crystallography grade NOV Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Nephroblastoma Overexpressed (NOV) (AA 22-354) protein (His tag)Crystallography grade NOV Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
-
Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "Roles of heterotypic CCN2/CTGF-CCN3/NOV and homotypic CCN2-CCN2 interactions in expression of the differentiated phenotype of chondrocytes." in: The FEBS journal, Vol. 279, Issue 19, pp. 3584-97, (2012) (PubMed).
: "Novel effects of CCN3 that may direct the differentiation of chondrocytes." in: FEBS letters, Vol. 585, Issue 19, pp. 3033-40, (2011) (PubMed).
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: "Roles of heterotypic CCN2/CTGF-CCN3/NOV and homotypic CCN2-CCN2 interactions in expression of the differentiated phenotype of chondrocytes." in: The FEBS journal, Vol. 279, Issue 19, pp. 3584-97, (2012) (PubMed).
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- Target
- NOV (Nephroblastoma Overexpressed (NOV))
- Alternative Name
- NOV (NOV Products)
- Synonyms
- CCN3 Protein, IBP-9 Protein, IGFBP-9 Protein, IGFBP9 Protein, NOVh Protein, nov-A Protein, xnov Protein, NovH Protein, C130088N23Rik Protein, nephroblastoma overexpressed Protein, nephroblastoma overexpressed L homeolog Protein, nephroblastoma overexpressed gene Protein, NOV Protein, nov.L Protein, Nov Protein
- Background
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Full Gene Name: nephroblastoma overexpressed gene
Synonyms: CCN3,IGFBP9 - Gene ID
- 4856
- Pathways
- Smooth Muscle Cell Migration, Growth Factor Binding
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