IL-2 Protein

Catalog No. ABIN7866432

This Recombinant IL-2 protein is produced in Escherichia coli (E. coli).

Quick Overview for IL-2 Protein (ABIN7866432)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 98 % by SDS-PAGE

Product Details

Purpose: Recombinant Human IL-2 Protein

Sequence: MAPTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN RWITFCQSII STLHYKDPAA NKARKEAELA AATAEQ

Specificity: Chromosomal location:4q26-q27

Characteristics: Length (aa):156

Endotoxin Level: < 0.1 ng per μg of IL-2

Application Details

Application Notes: The ED50 as determined by the dose-dependent stimulation of proliferation of murine D10G4.1 cells is in the range of 0.1-0.5 ng/mL.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 mM acetic acid,The lyophilized IL-2 should be reconstituted in 50 mM acetic acid to a concentration not less than 100 μg/mL. This solution can be diluted into other buffered solutions or stored at -20 °C for future use.

Buffer: 50 mM acetic acid

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 0 °C,-20 °C

Storage Comment: The lyophilized IL-2, though stable at room temperature, is best stored desiccated below 0°C. Reconstituted IL-2 should be stored in workingaliquots at -20°C. Avoid repeated freeze-thaw cycles.

Target Details

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: IL-2

Background:

Synonyms: IL2, IL-2, TCGF, lymphokine

Description: IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes. Recombinant human IL-2 (modified at the C-terminal end) is a 15.5 kDa protein, containing 156 amino acid residues including one intrachain disulfide bond.

Molecular Weight: 15.5 kDa

Gene ID: 3558

NCBI Accession: NM_000586, NP_000577

UniProt: P60568

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation