TIM Protein (His tag)

Catalog No. ABIN7959185

This Recombinant TIM protein is expressed in Escherichia coli (E. coli).

Quick Overview for TIM Protein (His tag) (ABIN7959185)

Target: TIM (Triosephosphate Isomerase (TIM))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: Greater than 95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This TIM protein is labelled with His tag.

Purpose: Recombinant Human Triosephosphate Isomerase/TIM (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MAPSRKFFVG GNWKMNGRKQ SLGELIGTLN AAKVPADTEV VCAPPTAYID FARQKLDPKI AVAAQNCYKV TNGAFTGEIS PGMIKDCGAT WVVLGHSERR HVFGESDELI GQKVAHALAE GLGVIACIGE KLDEREAGIT EKVVFEQTKV IADNVKDWSK VVLAYEPVWA IGTGKTATPQ QAQEVHEKLR GWLKSNVSDA VAQSTRIIYG GSVTGATCKE LASQPDVDGF LVGGASLKPE FVDIINAKQ

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.< br ,>It is not recommended to reconstitute to a concentration less than 100 μ,g,ml.< br ,>Dissolve the lyophilized protein in ddH2O.< br ,>Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 um filtered solution of 20 mM TrisHCl, 1 mM DTT, 10 % Glycerol, pH 8.0.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Please minimize freeze-thaw cycles

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: TIM (Triosephosphate Isomerase (TIM))

Alternative Name: Triosephosphate Isomerase/TIM

Molecular Weight: 28.8kDa

Gene ID: 7167

UniProt: P60174