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TIM Protein (AA 1-249) (His tag)

TIM Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by reducing SDS-PAGE.
Catalog No. ABIN1097652
  • Target See all TIM products
    TIM (Triosephosphate Isomerase (TIM))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-249
    Origin
    Human
    Source
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This TIM protein is labelled with His tag.
    Purpose
    Recombinant Human Triosephosphate Isomerase/TIM (N-6His)
    Sequence
    MGSSHHHHHH SSGLVPRGSH MAPSRKFFVG GNWKMNGRKQ SLGELIGTLN AAKVPADTEV VCAPPTAYID FARQKLDPKI AVAAQNCYKV TNGAFTGEIS PGMIKDCGAT WVVLGHSERR HVFGESDELI GQKVAHALAE GLGVIACIGE KLDEREAGIT EKVVFEQTKV IADNVKDWSK VVLAYEPVWA IGTGKTATPQ QAQEVHEKLR GWLKSNVSDA VAQSTRIIYG GSVTGATCKE LASQPDVDGF LVGGASLKPE FVDIINAKQ
    Characteristics
    Recombinant Human Triosephosphate Isomerase/TIM is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Gln249) of Human TPI1 fused with a His tag at the N-terminus.
    Purity
    > 95 % as determined by reducing SDS-PAGE.
    Sterility
    0.2 μm filtered
    Endotoxin Level
    Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
  • Restrictions
    For Research Use only
  • Format
    Liquid
    Reconstitution
    It is not recommended to reconstitute to a concentration less than 100 μg/mL.
    Dissolve the lyophilized protein in ddH2O.
    Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
    Buffer
    Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 1 mM DTT, 10 % Glycerol, pH 8.0.
    Preservative
    Dithiothreitol (DTT)
    Precaution of Use
    This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
    Storage
    -80 °C
    Storage Comment
    Store at < -20°C, stable for 6 months after receipt.
    Please minimize freeze-thaw cycles.
    Expiry Date
    6 months
  • Target
    TIM (Triosephosphate Isomerase (TIM))
    Alternative Name
    Triosephosphate Isomerase/TIM (TIM Products)
    Synonyms
    F26H11.7 Protein, F26H11_7 Protein, PDTPI Protein, PLASTID ISOFORM TRIOSE PHOSPHATE ISOMERASE Protein, TRIOSEPHOSPHATE ISOMERASE Protein, triosephosphate isomerase Protein, triosephosphate isomerase Protein, TIM Protein
    Sub Type
    Fusionprotein
    Background
    Triose-phosphate isomerase, also named Triose-phosphate isomerase, TPI and TIM, is an enzyme that catalyzes the reversible interconversion of the triose phosphate isomers dihydroxyacetone phosphate and D-glyceraldehyde 3-phosphate. TPI has been found in nearly every organism searched for the enzyme, including animals such as mammals and insects as well as in fungi, plants, and bacteria. However, some bacteria that do not perform glycolysis, like ureaplasmas, lack TPI. TPI plays an important role in glycolysis and is essential for efficient energy production. TPI deficiency is an autosomal recessive disorder and the most severe clinical disorder of glycolysis. Triose phosphate isomerase deficiency is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection and characterized by chronic hemolytic anemia.
    Alternative Names: Triosephosphate Isomerase, TIM, Triose-Phosphate Isomerase, TPI1, TPI
    Molecular Weight
    28.8 kDa
    UniProt
    P60174
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