IL-3 Protein
Quick Overview for IL-3 Protein (ABIN7956069)
Target
See all IL-3 ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Purpose
- Recombinant Rhesus Macaque Interleukin-3
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Sequence
- APMTQTTSLK TSWAKCSNMI DEIITHLNQP PLPSPDFNNL NEEDQTILVE KNLRRSNLEA FSKAVKSLQN ASAIESILKN LPPCLPMATA APTRPPIRIT NGDRNDFRRK LKFYLKTLEN EQAQ
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Less than 1 EU/ug of rRhIL-3 as determined by LAL method.
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Biological Activity Comment
- Assay #1: Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of murine NFS-60 cells is less than 5.0 ng/ml, corresponding to a specific activity of > 2 x105 IU/mg.Assay #2: The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 x107 IU/mg.
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Interleukin 3 (IL-3) (AA 20-152) (Active) protein (His-Avi Tag)
IL-3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Interleukin 3 (IL-3) protein (His tag)IL-3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by reducing SDS-PAGE. AM, ADA, IA, ScA
Interleukin 3 (IL-3) (AA 20-143) (Active) protein (His tag)IL-3 Origin: Cynomolgus Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Interleukin 3 (IL-3) (AA 20-152) (Active) protein (Biotin,His-Avi Tag)IL-3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Interleukin 3 (IL-3) (AA 27-166) (Active) protein (hFc Tag)IL-3 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Interleukin 3 (IL-3) (AA 33-166) (Active) proteinIL-3 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE analysis. Active
Interleukin 3 (IL-3) (AA 1-152) protein (GST tag)IL-3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Interleukin 3 (IL-3) protein (His tag)IL-3 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
Interleukin 3 (IL-3) (AA 20-152) (Active) proteinIL-3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Interleukin 3 (IL-3) (AA 33-166) (Active) protein (His tag)IL-3 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
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Comment
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Approximately 14.0 kDa, a single non-glycosylated polypeptide chain containing 124 amino acids.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
- Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, 5 % trehalose.
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Expiry Date
- 12 months
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- IL-3 (Interleukin 3 (IL-3))
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Alternative Name
- Interleukin-3
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Molecular Weight
- 14.0 kDa
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Gene ID
- 706946
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UniProt
- P25140
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Pathways
- JAK-STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy
Target
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