VEGFA Protein
Quick Overview for VEGFA Protein (ABIN7956284)
Target
See all VEGFA ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Purpose
- Recombinant Rat Vascular Endothelial Growth Factor 164, Yeast
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Sequence
- MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Less than 0.1 EU/ug of rRtVEGF164, Yeast as determined by LAL method.
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Biological Activity Comment
- Fully biologically active when compared to standard. Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 0.75-3.75 ng/ml.
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Vascular Endothelial Growth Factor A (VEGFA) (AA 27-147) protein (His tag)
VEGFA Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Vascular Endothelial Growth Factor A (VEGFA) (AA 27-147) protein (Fc Tag)VEGFA Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Vascular Endothelial Growth Factor A (VEGFA) (AA 1-147) protein (GST tag)VEGFA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Vascular Endothelial Growth Factor A (VEGFA) (AA 27-190) (Active) protein (His tag)VEGFA Origin: Mouse Host: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. WB, SDS, ELISA, Imm, AcA Active
Vascular Endothelial Growth Factor A (VEGFA) proteinVEGFA Origin: Mouse Host: Yeast (Pichia pastoris) Recombinant > 95 % as determined by SDS-PAGE
Vascular Endothelial Growth Factor A (VEGFA) proteinVEGFA Origin: Rat Host: Yeast Recombinant > 95 % as determined by SDS-PAGE
Vascular Endothelial Growth Factor A (VEGFA) (AA 27-146) protein (His tag)VEGFA Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Vascular Endothelial Growth Factor A (VEGFA) (AA 27-146) protein (His-IF2DI Tag)VEGFA Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Vascular Endothelial Growth Factor A (VEGFA) (AA 27-147) protein (His tag)VEGFA Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Vascular Endothelial Growth Factor A (VEGFA) (AA 27-146) (Active) protein (His tag,AVI tag,Biotin)VEGFA Origin: Mouse Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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Comment
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Theoretically as a disulfide-linked homodimeric protein, the product consists of two 165 amino acid polypeptide chains. As a result of glycosylation, it migrates to at least two bands with molecular weights ranging from 25.7 kDa in SDS-PAGE under reducing conditions.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
- Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
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Storage
- -20 °C
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Storage Comment
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Expiry Date
- 12 months
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- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
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Alternative Name
- Vascular Endothelial Growth Factor 164
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Molecular Weight
- 25.7 kDa
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Pathways
- RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
Target
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