CNTF Protein
Quick Overview for CNTF Protein (ABIN7937090)
Target
See all CNTF ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Purpose
- Recombinant Rat Ciliary Neurotrophic Factor
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Sequence
- AFAEQTPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNINLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LMLQVSAFAY QLEELMVLLE QKIPENEADG MPATVGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HQMGISALES HYGAKDKQM
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Less than 1 EU/ug of rRtCNTF as determined by LAL method.
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Biological Activity Comment
- Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 30 ng/ml, corresponding to a specific activity of > 3.3 x104 IU/mg.
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Ciliary Neurotrophic Factor (CNTF) (AA 1-200) protein (His tag)
custom-made CNTF Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ciliary Neurotrophic Factor (CNTF) (AA 1-198) protein (His tag)custom-made CNTF Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ciliary Neurotrophic Factor (CNTF) (AA 1-200) protein (GST tag)CNTF Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Ciliary Neurotrophic Factor (CNTF) (AA 1-200) protein (His-IF2DI Tag)CNTF Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Ciliary Neurotrophic Factor (CNTF) (AA 2-198) protein (His-IF2DI Tag)CNTF Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Ciliary Neurotrophic Factor (CNTF) (AA 1-200) protein (His tag)CNTF Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Ciliary Neurotrophic Factor (CNTF) (AA 1-200) (Active) protein (His tag)CNTF Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, AcA Active
Ciliary Neurotrophic Factor (CNTF) (AA 1-200) (Active) proteinCNTF Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS, AcA Active
Ciliary Neurotrophic Factor (CNTF) (AA 4-196), (partial) protein (His tag)CNTF Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
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Comment
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Approximately 22.7 kDa, a single non-glycosylated polypeptide chain containing 199 amino acids.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
- Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, pH 7.4, 2 % trehalose.
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Expiry Date
- 12 months
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- CNTF (Ciliary Neurotrophic Factor (CNTF))
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Alternative Name
- Ciliary Neurotrophic Factor
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Molecular Weight
- 22.7 kDa
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Gene ID
- 25707
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UniProt
- P20294
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Pathways
- JAK-STAT Signaling
Target
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