GDNF Protein

Catalog No. ABIN7956296

Recombinant GDNF protein expressed in Escherichia coli (E. coli).

Quick Overview for GDNF Protein (ABIN7956296)

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: Escherichia coli (E. coli)

Purity: > 98 % by SDS-PAGE and HPLC analyses.

Product Details

Purpose: Recombinant Rat Glial Cell-derived Neurotrophic Factor

Sequence: SPDKQAAALP RRERNRQAAA ASPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCEAAETMYD KILKNLSRSR RLTSDKVGQA CCRPVAFDDD LSFLDDSLVY HILRKHSAKR CGCI

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 EU/ug of rRtGDNF as determined by LAL method.

Biological Activity Comment: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 x10⁶ IU/mg.

Application Details

Comment:

Approximately 29.8 kDa, a homodimeric protein consisting of two 134 amino acid non-glycosylated polypeptide chains.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 1 x PBS, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Expiry Date: 12 months

Target Details

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Alternative Name: Glial Cell-derived Neurotrophic Factor

Molecular Weight: 29.8 kDa

Gene ID: 25453

UniProt: Q07731

Pathways: RTK Signaling, Synaptic Membrane, Tube Formation, Autophagy, Smooth Muscle Cell Migration