MANF Protein

Catalog No. ABIN7937203

Recombinant MANF protein expressed in Escherichia coli (E. coli).

Quick Overview for MANF Protein (ABIN7937203)

Target: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % by SDS-PAGE and HPLC analyses.

Product Details

Purpose: Recombinant Human Mesencephalic Astrocyte-Derived Neurotrophic Factor

Sequence: LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIENELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDKQ IDLSTVDLKK LRVKELKKIL DDWGETCKGC AEKSDYIRKI NELMPKYAPK AASARTDL

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 1 EU/ug of rHuMANF as determined by LAL method.

Biological Activity Comment: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 20 μg/ml, corresponding to a specific activity of > 50 IU/mg.

Application Details

Comment:

Approximately 18.2 kDa, a single non-glycosylated polypeptide chain containing158 amino acids.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Expiry Date: 12 months

Target Details

Target: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Alternative Name: Mesencephalic Astrocyte-Derived Neurotrophic Factor

Molecular Weight: 18.2 kDa

Gene ID: 7873

UniProt: P55145