LIF Protein
Quick Overview for LIF Protein (ABIN7937359)
Target
See all LIF ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Purpose
- Recombinant Human Leukemia Inhibitory Factor
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Sequence
- SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Less than 1 EU/ug of rHuLIF as determined by LAL method.
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Biological Activity Comment
- Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent proliferation of human TF-1 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 x107 IU/mg.
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Leukemia Inhibitory Factor (LIF) (AA 23-202) (Active) protein
LIF Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Leukemia Inhibitory Factor (LIF) (AA 24-203) (Active) proteinLIF Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Leukemia Inhibitory Factor (LIF) (AA 23-202) (Active) proteinLIF Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE analysis. Active
Leukemia Inhibitory Factor (LIF) (AA 25-203) (Active) protein (hFc Tag)LIF Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Leukemia Inhibitory Factor (LIF) (AA 1-202) protein (GST tag)LIF Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Leukemia Inhibitory Factor (LIF) (AA 24-203) (Active) protein (His tag)LIF Origin: Mouse Host: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. SDS, WB, ELISA, Imm, AcA Active
Leukemia Inhibitory Factor (LIF) (AA 23-202) (Active) protein (His tag)LIF Origin: Human Host: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. SDS, WB, ELISA, Imm, AcA Active
Leukemia Inhibitory Factor (LIF) (Active) proteinLIF Origin: Rat Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Leukemia Inhibitory Factor (LIF) (AA 23-202) protein (Fc Tag)LIF Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Leukemia Inhibitory Factor (LIF) (AA 23-202) (Active) protein (His tag)LIF Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
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Comment
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Approximately 19.7 kDa, a single non-glycosylated polypeptide chain containing 180 amino acids.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
- Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Expiry Date
- 12 months
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- LIF (Leukemia Inhibitory Factor (LIF))
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Alternative Name
- Leukemia Inhibitory Factor
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Molecular Weight
- 19.7 kDa
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Gene ID
- 3976
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UniProt
- P15018
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Pathways
- JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding
Target
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