LIF Protein
Quick Overview for LIF Protein (ABIN7937415)
Target
See all LIF ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Purpose
- Recombinant Murine Leukemia Inhibitory Factor
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Sequence
- SPLPITPVNA TCAIRHPCHG NLMNQIKNQL AQLNGSANAL FISYYTAQGE PFPNNVEKLC APNMTDFPSF HGNGTEKTKL VELYRMVAYL SASLTNITRD QKVLNPTAVS LQVKLNATID VMRGLLSNVL CRLCNKYRVG HVDVPPVPDH SDKEAFQRKK LGCQLLGTYK QVISVVVQAF
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Less than 1 EU/ug of rMuLIF as determined by LAL method.
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Biological Activity Comment
- Fully biologically active when compared to standard. The specific activity is determined by inducing differentiation of murine M1 myeloid leukemic cells. The minimum detectable concentration of rMuLIF in this assay is 0.01 ng/ml. The specific activity of > 1.0 x108 IU/mg, where 50 units is defined as the amount of rMuLIF required to induce differentiation in 50 % of the M1 colonies in 1 ml agar cultures.
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Leukemia Inhibitory Factor (LIF) (AA 23-202) (Active) protein
LIF Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Leukemia Inhibitory Factor (LIF) (AA 24-203) (Active) proteinLIF Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Leukemia Inhibitory Factor (LIF) (AA 23-202) (Active) proteinLIF Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE analysis. Active
Leukemia Inhibitory Factor (LIF) (AA 25-203) (Active) protein (hFc Tag)LIF Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Leukemia Inhibitory Factor (LIF) (AA 1-202) protein (GST tag)LIF Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Leukemia Inhibitory Factor (LIF) (AA 24-203) (Active) protein (His tag)LIF Origin: Mouse Host: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. SDS, WB, ELISA, Imm, AcA Active
Leukemia Inhibitory Factor (LIF) (AA 23-202) (Active) protein (His tag)LIF Origin: Human Host: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. SDS, WB, ELISA, Imm, AcA Active
Leukemia Inhibitory Factor (LIF) (Active) proteinLIF Origin: Rat Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Leukemia Inhibitory Factor (LIF) (AA 23-202) protein (Fc Tag)LIF Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Leukemia Inhibitory Factor (LIF) (AA 23-202) (Active) protein (His tag)LIF Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
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Comment
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Approximately 19.9 kDa, a single non-glycosylated polypeptide chain containing 180 amino acids.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
- Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, with 0.02 % TWEEN 20.
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Expiry Date
- 12 months
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- LIF (Leukemia Inhibitory Factor (LIF))
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Alternative Name
- Leukemia Inhibitory Factor
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Molecular Weight
- 19.9 kDa
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Gene ID
- 16878
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UniProt
- P09056
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Pathways
- JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding
Target
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