ALKBH1 Protein
Quick Overview for ALKBH1 Protein (ABIN7938582)
Target
See all ALKBH1 ProteinsProtein Type
Origin
Source
Application
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Purpose
- Recombinant ALKBH1 protein
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Characteristics
- ALKBH1 (AlkB Homolog 2, Alpha-Ketoglutarate Dependent Dioxygenase) is a member of ALKBH protein family. It can repair alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. ALKBH1 can also repair alkylated DNA containing 1-ethenoadenine (in vitro). Has strong preference for double-stranded DNA. It can release modified bases from both DNA and RNA. The oxidative demethylation requires molecular oxygen, alpha-ketoglutarate and iron.
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AlkB, Alkylation Repair Homolog 1 (ALKBH1) (AA 1-389) protein (His tag)
custom-made ALKBH1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
AlkB, Alkylation Repair Homolog 1 (ALKBH1) (AA 1-389) protein (His tag)custom-made ALKBH1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
AlkB, Alkylation Repair Homolog 1 (ALKBH1) (AA 1-389) protein (GST tag)ALKBH1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
AlkB, Alkylation Repair Homolog 1 (ALKBH1) protein (Myc-DYKDDDDK Tag)ALKBH1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Recombinant ALKBH1 protein is supplied in 25 mM Tris-HCl pH 8.0, 300 mM NaCl, 10 % glycerol, and 0.5 mM TCEP.
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Handling Advice
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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Storage
- -80 °C
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Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- ALKBH1 (AlkB, Alkylation Repair Homolog 1 (ALKBH1))
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Alternative Name
- ALKBH1
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Background
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Protein Background: ALKBH1 (AlkB Homolog 2, Alpha-Ketoglutarate Dependent Dioxygenase) is a member of ALKBH protein family. It can repair alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. ALKBH1 can also repair alkylated DNA containing 1-ethenoadenine (in vitro). Has strong preference for double-stranded DNA. It can release modified bases from both DNA and RNA. The oxidative demethylation requires molecular oxygen, alpha-ketoglutarate and iron.
Short Description: Full length ALKBH1 protein (accession number NP_006011.2) was expressed in E.coli cells with an N-terminal 6xHis Tag. The molecular weight of ALKBH1 is 47.6 kDa.
Aliases: AlkB Homolog 1, Histone H2A Dioxygenase
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Pathways
- DNA Damage Repair
Target
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