ACE2 Protein (AA 18-740)

Catalog No. ABIN7938599

Recombinant ACE2 protein expressed in Baculovirus infected Insect Cells.

Quick Overview for ACE2 Protein (AA 18-740) (ABIN7938599)

Target: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Functional Studies (Func), Screening Assay (ScA), Activity Assay (AcA)

Product Details

Protein Characteristics: AA 18-740

Purpose: Recombinant ACE2 (18-740) protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant ACE2 (18-740) protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, and 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Alternative Name: ACE2

Background:

Protein Background: ACE2 (Angiotensin I Converting Enzyme 2) belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases, it is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis. This protein catalyzes the cleavage of angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to the vasodilator angiotensin 1-7, which acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II. ACE2 removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin. Also cleaves other biological peptides, such as apelins (apelin-13, [Pyr1] apelin-13, apelin-17, apelin-36), casomorphins (beta-casomorphin-7, neocasomorphin) and dynorphin A with high efficiency. In addition, ACE2 C-terminus is homologous to collectrin and is responsible for the trafficking of the neutral amino acid transporter SL6A19 to the plasma membrane of gut epithelial cells via direct interaction, regulating its expression on the cell surface and its catalytic activity.

Short Description: Recombinant ACE2 (18-740) protein that includes amino acids 18-740 of human ACE2 protein (accession number NP_001358344.1) was expressed in a baculovirus expression system, and contains a C-terminal FLAG tag. The molecular weight of the protein is 85.4 kDa.

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour