Aurora Kinase B Protein (AURKB)

Catalog No. ABIN7938612

This Recombinant Aurora Kinase B protein is produced in Baculovirus infected Insect Cells.

Quick Overview for Aurora Kinase B Protein (AURKB) (ABIN7938612)

Target: Aurora Kinase B (AURKB)

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Functional Studies (Func), Activity Assay (AcA), Screening Assay (ScA)

Product Details

Purpose: Recombinant AURKB protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant AURKB protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, and 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: Aurora Kinase B (AURKB)

Alternative Name: AURKB

Background:

Protein Background: AURKB (Aurora Kinase B) is a member of the aurora kinase subfamily of serine/threonine kinases and phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity. The CPC complex acts as a key regulator of mitosis. which has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatininduced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Other known AURKB substrates involved in centromeric functions and mitosis are CENPA, DES/desmin, GPAF, KIF2C, NSUN2, RACGAP1, SEPTIN1, VIM/ vimentin, HASPIN, and histone H3. Phosphorylation of VIM controls vimentin filament segregation in cytokinetic process, whereas histone H3 is phosphorylated at 'Ser-10' and 'Ser-28' during mitosis (H3S10ph and H3S28ph, respectively). A positive feedback between HASPIN and AURKB contributes to CPC localization. AURKB is also required for kinetochore localization of BUB1 and SGO1.

Short Description: Recombinant AURKB protein was expressed in baculovirus expression system as the full length protein (accession number AAC12709.1) with a N-terminal FLAG tag. The molecular weight of the protein is 40.5 kDa.

Aliases: Aurora kinase B

Pathways: TCR Signaling, Cell Division Cycle, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades