BRD2 Protein

Catalog No. ABIN7938621

This Recombinant BRD2 protein is expressed in Escherichia coli (E. coli).

Quick Overview for BRD2 Protein (ABIN7938621)

Target: BRD2 (Bromodomain Containing 2 (BRD2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Screening Assay (ScA), Activity Assay (AcA), Functional Studies (Func)

Product Details

Purpose: Recombinant BRD2 (BD1+BD2) protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant BRD2 (BD1+BD2) protein is supplied in 25 mM Tris pH 8.0, 300 mM NaCl, 10 % glycerol, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: BRD2 (Bromodomain Containing 2 (BRD2))

Alternative Name: BRD2

Background:

Protein Background: Bromodomain-containing protein 2 (BRD2), also known as RING3, belongs to the BET subclass of proteins, which are characterized by two N-terminal bromodomains and one ET (Extra Terminal) domain. BRDs associate with chromatin through their bromodomains that recognize acetylated histone lysine residues. Bromodomains function as 'readers' of these epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. The ET domain functions as a protein binding motif and exerts atypical serine-kinase activity. The BET family consists of at least four members in mouse and human, BRD2 (also referred to as FSRG1, RING3), BRD3 (FSRG2, ORFX), BRD4 (FSRG4, MCAP/HUNK1), and BRDT (FSRG3, BRD6). BRD proteins are related to the female sterile homeotic protein gene in Drosophila, a gene required maternally for proper expression of other homeotic genes, such as Ubx, which is involved in pattern formation. BRD2 causes elevated protein kinase activity in leukemias. Transgenic mice overexpressing BRD2 in the lymphoid system develop diffuse large-cell lymphoma. BRD2 has been shown to interact with E2F1 and with histone H4 acetylated at Lys12 via its two bromodomains. BRD2 may play a role in spermatogenesis or folliculogenesis. Genetic evidence links the BRD2 gene to both juvenile myoclonic epilepsy and photoparoxysomal responses.

Short Description: The protein corresponding to amino acids 71-455 that contains the bromodomain sequence of BRD2 (accession number NP_001106653.1) was expressed in E. coli and contains an N-terminal His tag with an observed molecular weight of 45.51 kDa. It shows binding specificity for acetylated H4K5, H4K5/8, H4K5/12, H4K8/12, H4K12/16, H4K12/16/20 and H4K5/8/12/16

Aliases: FSH, NAT, RNF3, FSRG1, RING3, D6S113E

Pathways: Chromatin Binding, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection