CDK6/CyclinD3 (Complex) Protein

Catalog No. ABIN7938700

Recombinant CDK6/CyclinD3 protein expressed in Baculovirus infected Insect Cells.

Quick Overview for CDK6/CyclinD3 (Complex) Protein (ABIN7938700)

Target: CDK6/CyclinD3

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Functional Studies (Func), Activity Assay (AcA), Screening Assay (ScA)

Product Details

Protein Characteristics: Complex

Purpose: Recombinant CDK6 / Cyclin D3 Complex

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant CDK6 / Cyclin D3 Complex is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: CDK6/CyclinD3

Alternative Name: CDK6 & Cyclin D3

Background:

Protein Background: CDK6 is a serine/threonine-protein kinase involved in the control of the cell cycle and differentiation, promotes G1/S transition. Phosphorylates pRB/RB1 and NPM1. Interacts with D-type G1 cyclins during interphase at G1 to form a pRB/RB1 kinase and controls the entrance into the cell cycle. Involved in initiation and maintenance of cell cycle exit during cell differentiation, prevents cell proliferation and negatively regulates cell differentiation but is required for the proliferation of specific cell types (e.g. erythroid and hematopoietic cells). Essential for cell proliferation within the dentate gyrus of the hippocampus and the subventricular zone of the lateral ventricles. Required during thymocyte development Promotes the production of newborn neurons, probably by modulating G1 length. Promotes, at least in astrocytes, changes in patterns of gene expression, changes in the actin cytoskeleton including loss of stress fibers, and enhanced motility during cell differentiation. Prevents myeloid differentiation by interfering with RUNX1 and reducing its transcription transactivation activity but promotes proliferation of normal myeloid progenitors. Delays senescence. Promotes the proliferation of beta-cells in pancreatic islets of Langerhans. May play a role in the centrosome organization during the cell cycle phases.

Short Description: Recombinant CDK6 / Cyclin D3 Complex that includes full length human CDK6 protein (accession number NP_001250.1) with an N-terminal His tag and full-length human Cyclin D3 protein (accession number NP_001751.1) with an C-terminal FLAG tag was expressed in Hi5 cells. The molecular weights of CDK6 and Cyclin D3 are 40.03 kDa and 34.21 kDa, respectively.