EPH Receptor B1 Protein (EPHB1) (AA 565-984)

Catalog No. ABIN7938802

This Recombinant EPH Receptor B1 protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for EPH Receptor B1 Protein (EPHB1) (AA 565-984) (ABIN7938802)

Target: EPH Receptor B1 (EPHB1)

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Protein Characteristics: AA 565-984

Purpose: Recombinant EPHB1 (565-984) protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant EPHB1 (565-984) protein is supplied in 25 mM HEPESNaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100 and 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: EPH Receptor B1 (EPHB1)

Alternative Name: EPHB1

Background:

Protein Background: EPHB1(Ephrin type-B receptor 1) is a receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Cognate/functional ephrin ligands for this receptor include EFNB1, EFNB2 and EFNB3. During nervous system development, regulates retinal axon guidance redirecting ipsilaterally ventrotemporal retinal ganglion cells axons at the optic chiasm midline. This probably requires repulsive interaction with EFNB2. In the adult nervous system together with EFNB3, regulates chemotaxis, proliferation and polarity of the hippocampus neural progenitors. In addition to its role in axon guidance also plays an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and synapse formation. May also regulate angiogenesis. More generally, may play a role in targeted cell migration and adhesion. Upon activation by EFNB1 and probably other ephrin-B ligands activates the MAPK/ERK and the JNK signaling cascades to regulate cell migration and adhesion respectively. Involved in the maintenance of the pool of satellite cells (muscle stem cells) by promoting their selfrenewal and reducing their activation and differentiation (By similarity).

Short Description: Recombinant EPHB1 (565-984) protein that includes amino acids 565-984 of human EPHB1 protein (accession number NP_004432.1) was expressed in a baculovirus expression system, and contains an N-terminal FLAG tag. The molecular weight of the protein is 48.78 kDa.

Aliases: Ephrin type-B receptor 1, EPHT2, HEK6, ELK, NET, EK6, Eph Tyrosine Kinase

Pathways: RTK Signaling