EPH Receptor B3 Protein (EPHB3) (AA 585-998)

Catalog No. ABIN7938805

This Recombinant EPH Receptor B3 protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for EPH Receptor B3 Protein (EPHB3) (AA 585-998) (ABIN7938805)

Target: EPH Receptor B3 (EPHB3)

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Screening Assay (ScA), Functional Studies (Func), Activity Assay (AcA)

Product Details

Protein Characteristics: AA 585-998

Purpose: Recombinant EPHB3 (585-998) protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant EPHB3 (585-998) protein is supplied in 25 mM HEPESNaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100 and 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: EPH Receptor B3 (EPHB3)

Alternative Name: EPHB3

Background:

Protein Background: EPHB3(Ephrin type-B receptor 3) is a receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally, has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex. In addition to its role in axon guidance also plays an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt.

Short Description: Recombinant EPHB3 (585-998) protein that includes amino acids 585-998 of human EPHB3 protein (accession number NP_004434.2) was expressed in a baculovirus expression system, and contains an N-terminal FLAG tag. The molecular weight of the protein is 48.19 kDa.

Aliases: Ephrin type-B receptor 3, EPH Receptor B3, HEK2, TYRO6, ETK2, Tyrosine-Protein Kinase TYRO6, EK2, Tyro6, EphB3, Hek2

Pathways: RTK Signaling