Glucagon Protein (GCG) (His tag)

Catalog No. ABIN7938858

This Recombinant Glucagon protein is expressed in Escherichia coli (E. coli).

Quick Overview for Glucagon Protein (GCG) (His tag) (ABIN7938858)

Target: Glucagon (GCG)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Purification tag / Conjugate: This Glucagon protein is labelled with His tag.

Purpose: Recombinant GLU protein

Characteristics: GLU protein was expressed in E. coli (accession number NP_001028216.1) with a C-terminal 6xHis Tag. The molecular weight of the protein is 43.1 kDa.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant GLU protein is supplied in 25 mM Tris pH 8.0, 300 mM NaCl, 10 % glycerol, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: Glucagon (GCG)

Alternative Name: GLU

Background:

Protein Background: GLU (Glutamine synthetase), also called as GLU, is an enzymatic that catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants.

Short Description: GLU protein was expressed in E. coli cells (accession number NP_001028216.1) with a C-terminal 6xHis Tag. The molecular weight of the protein is 43.1 kDa.

Aliases: Glutamine synthetase

Molecular Weight: 43.1 kDa

NCBI Accession: NP_001028216

Pathways: Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Negative Regulation of intrinsic apoptotic Signaling