GNMT Protein
Quick Overview for GNMT Protein (ABIN7938868)
Target
See all GNMT ProteinsProtein Type
Origin
Source
Application
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Purpose
- Recombinant GNMT protein
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Glycine N-Methyltransferase (GNMT) (AA 1-295) protein (GST tag)
GNMT Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Glycine N-Methyltransferase (GNMT) (AA 1-295) (Active) protein (His tag)GNMT Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, EAA Active
Glycine N-Methyltransferase (GNMT) protein (Myc-DYKDDDDK Tag)GNMT Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Recombinant GNMT protein is supplied in 25 mM Tris pH 8.0, 300 mM NaCl, 10 % glycerol, 0.5 mM TCEP.
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Handling Advice
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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Storage
- -80 °C
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Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- GNMT (Glycine N-Methyltransferase (GNMT))
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Alternative Name
- GNMT
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Background
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Protein Background: Glycine N-methyltransferase (GNMT) catalyzes the transfer of a methyl group from S-adenosylmethionine (SAM) to glycine to yield S-adenosylhomocysteine (SAH) and N-methylglycine (sarcosine). The enzyme appears to play a significant role in maintaining the physiologically appropriate SAM-to-SAH ratio, which is thought to be a critical component of the regulation of the methylation potential of the cell.
Short Description: Recombinant GNMT protein was expressed in E.coli as the full length protein (accession number NP_061833.1) with an N-terminal 6xHis tag. The molecular weight of the protein is 34.54 kDa.
Aliases: Glycine N-methyltransferase
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Pathways
- Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process
Target
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