HIPK2 Protein (AA 1-640)

Catalog No. ABIN7939162

This Recombinant HIPK2 protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for HIPK2 Protein (AA 1-640) (ABIN7939162)

Target: HIPK2 (Homeodomain Interacting Protein Kinase 2 (HIPK2))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Functional Studies (Func), Activity Assay (AcA), Screening Assay (ScA)

Product Details

Protein Characteristics: AA 1-640

Purpose: Recombinant HIPK2 (1-640) protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant HIPK2 (1-640) protein is supplied in 25 mM HEPES-NaOH pH 7.5, 500 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, and 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: HIPK2 (Homeodomain Interacting Protein Kinase 2 (HIPK2))

Alternative Name: HIPK2

Background:

Protein Background: HIPK2 (Homeodomain Interacting Protein Kinase 2) is a conserved serine/threonine kinase that involved in transcription regulation, p53/TP53-mediated cellular apoptosis and regulation of the cell cycle. It acts as a corepressor of several transcription factors, including SMAD1 and POU4F1/Brn3a and probably NK homeodomain transcription factors. HIPK2 phosphorylates PDX1, ATF1, PML, p53/TP53, CREB1, CTBP1, CBX4, RUNX1, EP300, CTNNB1, HMGA1 and ZBTB4. It inhibits cell growth and promotes apoptosis through the activation of p53/TP53 both at the transcription level and at the protein level (by phosphorylation and indirect acetylation), the phosphorylation of p53/TP53 may be mediated by a p53/TP53-HIPK2-AXIN1 complex.

Short Description: Recombinant HIPK2 (1-640) protein that includes amino acids 1-640 of human HIPK2 protein (accession number NP_001106710.1) was expressed in a baculovirus expression system, and contains an N-terminal FLAG tag. The molecular weight of the protein is 69.5 kDa.

Aliases: PRO0593

Pathways: Cell Division Cycle