MBD2 Protein (Methyl-CpG-binding domain)

Catalog No. ABIN7939273

Recombinant MBD2 protein expressed in Escherichia coli (E. coli).

Quick Overview for MBD2 Protein (Methyl-CpG-binding domain) (ABIN7939273)

Target: MBD2 (Methyl-CpG Binding Domain Protein 2 (MBD2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Protein Characteristics: Methyl-CpG-binding domain

Purpose: Recombinant MBD2-MBD protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant MBD2-MBD protein is supplied in 25 mM Tris 8.0, 300 mM NaCl, 20 % glycerol, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: MBD2 (Methyl-CpG Binding Domain Protein 2 (MBD2))

Alternative Name: MBD2

Background:

Protein Background: MBD2 (Methyl-CpG-binding domain protein 2)is a transcriptional repressor that Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Also binds hemimethylated DNA as well and recruits histone deacetylases and DNA methyltransferases to chromatin. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.

Short Description: Recombinant MBD2-MBD protein that includes amino acids 140-219 of human MBD2 protein (accession number NP_003918.1) was expressed in E. coli cells with an N-terminal 6xHis tag. The molecular weight of the protein is 11.19 kDa.

Aliases: Methyl-CpG -binding domain protein 2, CXXC3, PCM1, Protein Containing Methyl-CpG Binding Domain 1

Pathways: Chromatin Binding