MAPKAP Kinase 3 Protein

Catalog No. ABIN7939342

This Recombinant MAPKAP Kinase 3 protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for MAPKAP Kinase 3 Protein (ABIN7939342)

Target: MAPKAP Kinase 3 (MAPKAPK3) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 3 (MAPKAPK3))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Functional Studies (Func), Activity Assay (AcA), Screening Assay (ScA)

Product Details

Purpose: Recombinant MAPKAPK3 protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant MAPKAPK3 protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, and 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: MAPKAP Kinase 3 (MAPKAPK3) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 3 (MAPKAPK3))

Alternative Name: MAPKAPK3

Background:

Protein Background: MAPKAPK3 (MAPK Activated Protein Kinase 3) is a mitogen-activated protein kinase (MAP kinase)-activated protein kinase which involved in cytokines production, endocytosis, cell migration, chromatin remodeling and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, which leading to phosphorylation of substrates. MAPKAPK3 phosphorylates serine in the peptide sequence, Hyd-X-RX(2)-S, where Hyd is a large hydrophobic residue and mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. It involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins, such as TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, and promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. It involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3 and also acts as a modulator of Polycomb-mediated repression. Diseases associated with MAPKAPK3 include Macular Dystrophy, Patterned, 3 and Bacteremia 2.

Short Description: Recombinant MAPKAPK3 protein was expressed in baculovirus expression system as the full length protein (accession number NP_001230854.1) with a N-terminal FLAG tag. The molecular weight of the protein is 44 kDa

Aliases: MAPK Activated Protein Kinase 3, MK-3, Mitogen-Activated Protein Kinase-Activated Protein Kinase 3

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades