PARG Protein (PARG)

Catalog No. ABIN7939405

Recombinant PARG protein expressed in Baculovirus infected Insect Cells.

Quick Overview for PARG Protein (PARG) (ABIN7939405)

Target: PARG

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Purpose: Recombinant PARG protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant PARG protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: PARG

Alternative Name: PARG

Background:

Protein Background: PARG (Poly (ADP-ribose) glycohydrolase) catalyzes the hydrolysis of glycosidic bonds of ADP-ribose polymers, producing monomeric ADP-ribose units. Thus, in conjunction with poly(ADP-ribose) polymerase (PARP), PARG activity regulates the extent of in vivo poly(ADP-ribosyl)ation. PARG acts both as an endo- and exoglycosidase, releasing poly (ADP-ribose) of different lengths as well as ADP-ribose monomers. Involved in the synthesis of ATP in the nucleus, together with PARP1, NMNAT1 and NUDT5.

Short Description: Recombinant PARG protein that includes full length of human PARG protein (accession number NP_003622.2) was expressed in Sf9 cells and contains an C-terminal His and FLAG tag with a molecular weight of 113.63 kDa. The purity of the protein is ≥ 75 % by SDS-PAGE.

Aliases: Poly(ADP-ribose) glycohydrolase, PARG, Mitochondrial Poly(ADP-Ribose) Glycohydrolase