PDGFRA Protein (AA 550-1089)

Catalog No. ABIN7939422

This Recombinant PDGFRA protein is produced in Baculovirus infected Insect Cells.

Quick Overview for PDGFRA Protein (AA 550-1089) (ABIN7939422)

Target: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Functional Studies (Func), Activity Assay (AcA), Screening Assay (ScA)

Product Details

Protein Characteristics: AA 550-1089

Purpose: Recombinant PDGFRA (550-1089) protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant PDGFRA (550-1089) protein is supplied in 25 mM HEPES NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Alternative Name: PDGFRA

Background:

Protein Background: PDGFRA (Platelet Derived Growth Factor Receptor Alpha), a tyrosine-protein kinase that acts as a cell surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival, and chemotaxis. Depending on the context, PDGFRA promotes or inhibits cell proliferation and cell migration and plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. PDGFRA phosphorylates PIK3R1, PLCG1, and PTPN11. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca (2+) and the activation of protein kinase C. PDGFRA phosphorylates PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and thereby mediates activation of the AKT1 signaling pathway. This protein also mediates activation of HRAS and of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1 and promotes activation of STAT family members STAT1, STAT3 and STAT5A and/or STAT5B.

Short Description: Recombinant PDGFRA (550-1089) protein that includes amino acids 550-1089 of human PDGFRA protein (accession number NP_001334758.1) was expressed in a baculovirus expression system, and contains an N-terminal FLAG tag. The molecular weight of the protein is 62.8 kDa.

Aliases: Platelet Derived Growth Factor Receptor Alpha

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Platelet-derived growth Factor Receptor Signaling