PLK3 Protein (AA 57-340)

Catalog No. ABIN7939428

This Recombinant PLK3 protein is produced in Baculovirus infected Insect Cells.

Quick Overview for PLK3 Protein (AA 57-340) (ABIN7939428)

Target: PLK3 (Polo-Like Kinase 3 (PLK3))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Functional Studies (Func), Activity Assay (AcA), Screening Assay (ScA)

Product Details

Protein Characteristics: AA 57-340

Purpose: Recombinant PLK3 (57-340) protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant PLK3 (57-340) protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, and 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: PLK3 (Polo-Like Kinase 3 (PLK3))

Alternative Name: PLK3

Background:

Protein Background: PLK3 (Polo Like Kinase 3) is a serine/threonine-protein kinase involved in cell cycle regulation, response to stress and Golgi disassembly and phosphorylates ATF2, BCL2L1, CDC25A, CDC25C, CHEK2, HIF1A, JUN, p53/ TP53, p73/TP73, PTEN, TOP2A and VRK1. PLK3 phosphorylates BCL2L1, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis and plays a key role in response to stress: rapidly activated upon stress stimulation, such as ionizing radiation, reactive oxygen species (ROS), hyperosmotic stress, UV irradiation and hypoxia. PLK3 involved in DNA damage response and G1/S transition checkpoint by phosphorylating CDC25A, p53/TP53 and p73/TP73 and phosphorylates p53/TP53 in response to reactive oxygen species (ROS), thereby promoting p53/TP53- mediated apoptosis and phosphorylates CHEK2 in response to DNA damage, promoting the G2/M transition checkpoint. It phosphorylates the transcription factor p73/TP73 in response to DNA damage, leading to inhibit p73/TP73-mediated transcriptional activation and pro-apoptotic functions and phosphorylates HIF1A and JUN is response to hypoxia and phosphorylates ATF2 following hyperosmotic stress in corneal epithelium. Diseases associated with PLK3 include Breast Cancer.

Short Description: Recombinant PLK3 (57-340) protein that includes amino acids 57-340 of human PLK3 protein (accession number NP_004064.2) was expressed in a baculovirus expression system, and contains an N-terminal FLAG tag. The molecular weight of the protein is 33.7 kDa.

Aliases: Polo Like Kinase 3

Pathways: Regulation of long-term Neuronal Synaptic Plasticity