PKC delta Protein

Catalog No. ABIN7939466

This Recombinant PKC delta protein is produced in Baculovirus infected Insect Cells.

Quick Overview for PKC delta Protein (ABIN7939466)

Target: PKC delta (PKCd) (Protein Kinase C, delta (PKCd))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Functional Studies (Func), Activity Assay (AcA), Screening Assay (ScA)

Product Details

Purpose: Recombinant PRKCD protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant PRKCD protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: PKC delta (PKCd) (Protein Kinase C, delta (PKCd))

Alternative Name: PRKCD

Background:

Protein Background: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PRKCD (Protein Kinase C Delta), a calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays contrasting roles in cell death and cell survival by functioning as a pro-apoptotic protein during DNA damage-induced apoptosis, but acting as an anti-apoptotic protein during cytokine receptor-initiated cell death. PRKCD is involved in tumor suppression as well as survival of several cancers and is required for oxygen radical production by NADPH oxidase and acts as positive or negative regulator in platelet functional responses. PRKCD negatively regulates B cell proliferation and also has an important function in self-antigen induced B cell tolerance induction. Upon DNA damage, PRKCD activates the promoter of the death-promoting transcription factor BCLAF1/Btf to trigger BCLAF1-mediated p53/TP53 gene transcription and apoptosis. Diseases associated with PRKCD include Autoimmune Lymphoproliferative Syndrome, Type Iii and Autoimmune Lymphoproliferative Syndrome.

Short Description: Recombinant PRKCD protein was expressed in baculovirus expression system as the full length protein (accession number NP_001303256.1) with a N-terminal FLAG tag. The molecular weight of the protein is 78.8 kDa.

Aliases: Protein kinase C, PKC, Protein kinase C delta

Pathways: Interferon-gamma Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Dicarboxylic Acid Transport, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, Lipid Metabolism