RPS6KA3 Protein

Catalog No. ABIN7939517

Recombinant RPS6KA3 protein expressed in Baculovirus infected Insect Cells.

Quick Overview for RPS6KA3 Protein (ABIN7939517)

Target: RPS6KA3 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3 (RPS6KA3))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Functional Studies (Func), Activity Assay (AcA), Screening Assay (ScA)

Product Details

Purpose: Recombinant RPS6KA3/MAPKAPK1B protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant RPS6KA3 / MAPKAPK1B protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, and 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: RPS6KA3 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3 (RPS6KA3))

Alternative Name: RPS6KA3 & MAPKAPK1B

Background:

Protein Background: RPS6KA3 also known as MAPKAPK1B is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signaling pathway. It acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77. RPS6KA3 also regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1.Diseases associated with RPS6KA3 include Coffin-Lowry Syndrome and Symptomatic Form Of Coffin-Lowry Syndrome In Female Carriers. Among its related pathways are Cellular Senescence (REACTOME) and Activated TLR4 signaling.

Short Description: Recombinant RPS6KA3 / MAPKAPK1B protein was expressed in a baculovirus expression system as the full length protein (accession number NP_004577.1) with a N-terminal FLAG tag. The molecular weight of the protein is 84.7 kDa.

Aliases: RPS6KA3, ISPK1, MAPKAPK1B, RSK2, Ribosomal protein S6 kinase alpha-3, 90kD ribosomal protein S6 kinase 3, Insulin-stimulated protein kinase 1, MAP kinase-activated protein kinase 1b, Ribosomal S6 kinase 2, pp90RSK2

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades