RNMT Protein
Quick Overview for RNMT Protein (ABIN7939522)
Target
See all RNMT ProteinsProtein Type
Origin
Source
Application
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Purpose
- Recombinant RNMT protein
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RNA Guanine-7 Methyltransferase (RNMT) (AA 1-476) protein (GST tag)
RNMT Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
RNA Guanine-7 Methyltransferase (RNMT) (AA 1-476) protein (His tag)RNMT Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
RNA Guanine-7 Methyltransferase (RNMT) (AA 31-126) protein (His-IF2DI Tag)RNMT Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
RNA Guanine-7 Methyltransferase (RNMT) (AA 1-476) protein (His-IF2DI Tag)RNMT Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
RNA Guanine-7 Methyltransferase (RNMT) protein (Myc-DYKDDDDK Tag)RNMT Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Recombinant RNMT protein is supplied in 25 mM Tris-HCl pH 8.0, 300 mM NaCl, 10 % glycerol and 0.5 mM TCEP.
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Handling Advice
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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Storage
- -80 °C
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Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- RNMT (RNA Guanine-7 Methyltransferase (RNMT))
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Alternative Name
- RNMT
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Background
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Protein Background: RNMT(mRNA cap guanine-N7 methyltransferase) is a Catalytic subunit of the mRNA-capping methyltransferase RNMT:RAMAC complex that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs.Binds RNA containing 5'-terminal GpppC.
Short Description: Recombinant RNMT protein that includes full length of human RNMT protein (accession number NP_001365064.1) was expressed was expressed in E. coli cells with an N-terminal GST tag. The molecular weight of the protein is 81.68 kDa.
Aliases: mRNA cap guanine-N7 methyltransferase, RG7MT1, MRNA Cap Methyltransferase, N7-MTase, HCMT1, HMet, CMT1
Target
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