LKB1 Protein

Catalog No. ABIN7939544

Recombinant LKB1 protein expressed in Baculovirus infected Insect Cells.

Quick Overview for LKB1 Protein (ABIN7939544)

Target: LKB1 (STK11) (serine/threonine Kinase 11 (STK11))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Purpose: Recombinant STK11 / LKB1 protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant STK11 / LKB1 protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: LKB1 (STK11) (serine/threonine Kinase 11 (STK11))

Alternative Name: STK11 & LKB1

Background:

Protein Background: STK11 (Serine/Threonine Kinase 11) is a tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members and plays a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. STK11 phosphorylates the T-loop of AMPK family proteins, thus promoting their activity. STK11 phosphorylates PRKAA1, PRKAA2, BRSK1, BRSK2, MARK1, MARK2, MARK3, MARK4, NUAK1, NUAK2, SIK1, SIK2, SIK3 and SNRK but not MELK and also phosphorylates non-AMPK family proteins such as STRADA, PTEN and possibly p53/TP53. STK11 acts as a key upstream regulator of AMPK by mediating phosphorylation and activation of AMPK catalytic subunits PRKAA1 and PRKAA2 and thereby regulates processes including: inhibition of signaling pathways that promote cell growth and proliferation when energy levels are low, glucose homeostasis in liver, activation of autophagy when cells undergo nutrient deprivation, and B-cell differentiation in the germinal center in response to DNA damage.

Short Description: Recombinant STK11 / LKB1 protein was expressed in a baculovirus expression system as the full length protein (accession number NP_000446.1) with an N-terminal FLAG tag. The molecular weight of the protein is 49.6 kDa.

Aliases: Serine / threonine kinase 11

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect