TARDBP Protein (AA 2-414)

Catalog No. ABIN7939636

Recombinant TARDBP protein expressed in Escherichia coli (E. coli).

Quick Overview for TARDBP Protein (AA 2-414) (ABIN7939636)

Target: TARDBP (TAR DNA Binding Protein (TARDBP))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Protein Characteristics: AA 2-414

Purpose: Recombinant TARDBP (2-414) protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant TARDBP (2-414) protein is supplied in 25 mM Tris 8.0, 300 mM NaCl, 20 % glycerol, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: TARDBP (TAR DNA Binding Protein (TARDBP))

Alternative Name: TARDBP

Background:

Protein Background: TARDBP (TAR DNA-binding protein 43) also known as ALS10 and TDP-43. TARDBP is a RNA-binding protein that is involved in various steps of RNA biogenesis and processing. Preferentially binds, via its two RNA recognition motifs RRM1 and RRM2, to GU-repeats on RNA molecules predominantly localized within long introns and in the 3'UTR of mRNAs. In turn, regulates the splicing of many non-coding and protein-coding RNAs including proteins involved in neuronal survival, as well as mRNAs that encode proteins relevant for neurodegenerative diseases. In addition, TARDBP also has a variety of biological functions, such as plays a role in maintaining mitochondrial homeostasis by regulating the processing of mitochondrial transcripts, regulates also mRNA stability, participates also in the normal skeletal muscle formation and regeneration, negatively regulates the expression of CDK6 and regulates the expression of HDAC6, ATG7 and VCP in a PPIA/CYPA-dependent manner.

Short Description: Recombinant TARDBP (2-414) protein that includes amino acids 2-414 of human TARDBP protein (accession number NP_031401.1) was expressed in E. coli and contains an N-terminal His and FLAG tag with a molecular weight of 47.9 kDa. The purity of the protein is ≥ 60 % by SDS-PAGE.

Aliases: TAR DNA-binding protein 43, ALS10, TDP-43, TDP43

Pathways: Positive Regulation of Peptide Hormone Secretion