UBE2H Protein
Quick Overview for UBE2H Protein (ABIN7939700)
Target
See all UBE2H ProteinsProtein Type
Origin
Source
Application
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Purpose
- Recombinant UBE2H protein
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Ubiquitin-Conjugating Enzyme E2H (UBE2H) (AA 1-183) protein (GST tag)
UBE2H Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Ubiquitin-Conjugating Enzyme E2H (UBE2H) (AA 1-183) protein (His-IF2DI Tag)UBE2H Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Ubiquitin-Conjugating Enzyme E2H (UBE2H) (Transcript Variant 1) protein (His tag)UBE2H Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Ubiquitin-Conjugating Enzyme E2H (UBE2H) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)UBE2H Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Ubiquitin-Conjugating Enzyme E2H (UBE2H) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)UBE2H Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Recombinant UBE2H protein is supplied in 25 mM Tris 8.0, 300 mM NaCl, 20 % glycerol, 0.5 mM TCEP.
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Handling Advice
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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Storage
- -80 °C
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Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- UBE2H (Ubiquitin-Conjugating Enzyme E2H (UBE2H))
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Alternative Name
- UBE2H
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Background
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Protein Background: UBE2H (Ubiquitin-conjugating enzyme E2 H) also known as E2-20K, GID3, UBC8, UBCH, UBCH2.UBE2H is a member of the E2 ubiquitin-conjugating enzyme family which is required for post-replicative DNA damage repair and plays an important role in various cellular processes. UBE2H can accept ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. E2 ubiquitin conjugating enzyme that transfers ubiquitin to MAEA, a core component of the CTLH E3 ubiquitin-protein ligase complex. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Capable, in vitro, to ubiquitinate histone H2A.
Short Description: Recombinant UBE2H protein that includes full length of human UBE2H protein (accession number NP_003335.1) was expressed in E. coli and contains an N-terminal His tag with a molecular weight of 22.82 kDa. The purity of the protein is ≥ 95 % by SDS-PAGE.
Aliases: Ubiquitin conjugating enzyme E2 H, ubiquitin carrier protein H, E2-20K, GID3, UBC8, UBCH, UBCH2
Target
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