UBE2I Protein
Quick Overview for UBE2I Protein (ABIN7939702)
Target
See all UBE2I ProteinsProtein Type
Origin
Source
Application
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Purpose
- Recombinant UBE2I protein
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Ubiquitin-Conjugating Enzyme E2I (UBE2I) (AA 1-158) protein (GST tag)
UBE2I Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Ubiquitin-Conjugating Enzyme E2I (UBE2I) (AA 1-157), (partial) protein (GST tag)UBE2I Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
Ubiquitin-Conjugating Enzyme E2I (UBE2I) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)UBE2I Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Ubiquitin-Conjugating Enzyme E2I (UBE2I) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)UBE2I Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Recombinant UBE2I protein is supplied in 25 mM Tris 8.0, 300 mM NaCl, 20 % glycerol, 0.5 mM TCEP.
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Handling Advice
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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Storage
- -80 °C
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Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))
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Alternative Name
- UBE2I
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Background
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Protein Background: UBE2I (SUMO-conjugating enzyme UBC9) also known as C358B7.1, P18, UBC9. UBE2I is a member of the E2 ubiquitin-conjugating enzyme family which is required for post-replicative DNA damage repair and plays an important role in various cellular processes. UBE2I can accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4 and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'. Mediates sumoylation of ERCC6 which is essential for its transcription-coupled nucleotide excision repair activity.
Short Description: Recombinant UBE2I protein that includes full length of human UBE2I protein (accession number NP_003336.1) was expressed in E. coli and contains an N-terminal His tag with a molecular weight of 20.17 kDa. The purity of the protein is ≥ 95 % by SDS-PAGE.
Aliases: Ubiquitin conjugating enzyme E2 I, SUMO conjugating enzyme UBC9, C358B7.1, P18, UBC9, ubiquitin carrier protein 9, SUMO protein ligase, P18
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Pathways
- Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway
Target
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