UBE2W Protein
Quick Overview for UBE2W Protein (ABIN7939721)
Target
See all UBE2W ProteinsProtein Type
Origin
Source
Application
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Purpose
- Recombinant UBE2W protein
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Ubiquitin-Conjugating Enzyme E2W (UBE2W) (AA 1-151) protein (His tag)
custom-made UBE2W Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ubiquitin-Conjugating Enzyme E2W (UBE2W) (AA 1-159) protein (GST tag)UBE2W Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Ubiquitin-Conjugating Enzyme E2W (UBE2W) (AA 1-151) protein (His tag)UBE2W Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Ubiquitin-Conjugating Enzyme E2W (UBE2W) (AA 1-162) protein (His-IF2DI Tag)UBE2W Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Ubiquitin-Conjugating Enzyme E2W (UBE2W) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)UBE2W Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Recombinant UBE2W protein is supplied in 25 mM Tris pH 8.0, 300 mM NaCl, 20 % glycerol, 0.5 mM TCEP.
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Handling Advice
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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Storage
- -80 °C
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Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- UBE2W (Ubiquitin-Conjugating Enzyme E2W (UBE2W))
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Alternative Name
- UBE2W
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Background
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Protein Background: UBE2W (Ubiquitin-conjugating enzyme E2 W) is a member of the E2 ubiquitin-conjugating enzyme family which is required for postreplicative DNA damage repair and plays an important role in various cellular processes. UBE2W can accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Specifically monoubiquitinates the N-terminus of various substrates, including ATXN3, MAPT/TAU, POLR2H/RPB8 and STUB1/CHIP, by recognizing backbone atoms of disordered N-termini. Involved in degradation of misfolded chaperone substrates by mediating monoubiquitination of STUB1/CHIP, leading to recruitment of ATXN3 to monoubiquitinated STUB1/CHIP, and restriction of the length of ubiquitin chain attached to STUB1/CHIP substrates by ATXN3. After UV irradiation, but not after mitomycin-C (MMC) treatment, acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway.
Short Description: Recombinant UBE2W protein that includes full length of human UBE2W protein (accession number NP_060769.5) was expressed in E. coli and contains an N-terminal His tag with a molecular weight of 19.49 kDa. The purity of the protein is ≥ 95 % by SDS-PAGE.
Aliases: Ubiquitin conjugating enzyme E2 W, UBC-16
Target
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