GLI1 Protein
Quick Overview for GLI1 Protein (ABIN7939787)
Target
See all GLI1 ProteinsProtein Type
Origin
Source
Application
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Purpose
- Recombinant GLI1 protein
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Zinc Finger Protein GLI1 (GLI1) (AA 401-500) protein (GST tag)
GLI1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Zinc Finger Protein GLI1 (GLI1) protein (Myc-DYKDDDDK Tag)GLI1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Zinc Finger Protein GLI1 (GLI1) (AA 921-1106), (partial) protein (His tag)GLI1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. SDS
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Recombinant GLI1 protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.
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Handling Advice
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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Storage
- -80 °C
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Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- GLI1 (Zinc Finger Protein GLI1 (GLI1))
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Alternative Name
- GLI1
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Background
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Protein Background: GLI1 (Zinc finger protein GLI1) acts as a transcriptional activator. Binds to the DNA consensus sequence 5'-GACCACCCA-3'. Regulates the transcription of specific genes during normal development. Plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling. Plays a role in cell proliferation and differentiation via its role in SHH signaling.
Short Description: Recombinant GLI1 protein was expressed in a baculovirus expression system as the full length protein (accession number NP_005260.1) with an C-terminal FLAG tag. The molecular weight of GLI1 is 119.6 kDa.
Aliases: GLI, Zinc Finger Protein GLI1, PAPA8, PPD1
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Pathways
- Hedgehog Signaling, Dopaminergic Neurogenesis
Target
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