Luciferase Protein (AA 1-545) (His tag)
Quick Overview for Luciferase Protein (AA 1-545) (His tag) (ABIN8057081)
Target
See all Luciferase ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-545
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Purification tag / Conjugate
- This Luciferase protein is labelled with His tag.
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Purpose
- Recombinant Pennsylania firefly Luciferase Protein(His Tag)
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Sequence
- Met1-Leu545
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- < 10 EU/mg of the protein as determined by the LAL method
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Biological Activity Comment
- Not validated for activity
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Luciferase (AA 1-311) (Active) protein (His tag)
Origin: Sea pansy Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, EAA Active
Luciferase (AA 1-550) protein (His tag)Origin: Firefly Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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Buffer
- Lyophilized from a 0.2 μm filtered solution in PBS with 5 % Trehalose and 5 % Mannitol.
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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Expiry Date
- 12 months
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- Luciferase
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Molecular Weight
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Calculated MW: 59.8 kDa
Observed MW: 60 kDa
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UniProt
- Q27757
Target
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