LIF Protein (AA 25-202)
Quick Overview for LIF Protein (AA 25-202) (ABIN8128630)
Target
See all LIF ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 25-202
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Purpose
- Active Leukemia Inhibitory Factor (LIF)
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Sequence
- Leu25-Phe202
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Leukemia Inhibitory Factor (LIF) (AA 23-202) (Active) protein
LIF Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Leukemia Inhibitory Factor (LIF) (AA 24-203) (Active) proteinLIF Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Leukemia Inhibitory Factor (LIF) (AA 23-202) (Active) proteinLIF Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE analysis. Active
Leukemia Inhibitory Factor (LIF) (AA 25-203) (Active) protein (hFc Tag)LIF Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Leukemia Inhibitory Factor (LIF) (AA 24-203) (Active) protein (His tag)LIF Origin: Mouse Host: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. SDS, WB, Imm, ELISA, AcA Active
Leukemia Inhibitory Factor (LIF) (AA 23-202) (Active) protein (His tag)LIF Origin: Human Host: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. SDS, WB, Imm, ELISA, AcA Active
Leukemia Inhibitory Factor (LIF) (AA 23-202) protein (Fc Tag)LIF Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Leukemia Inhibitory Factor (LIF) (AA 23-202) (Active) protein (His tag)LIF Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90 % SDS, AcA Active
Leukemia Inhibitory Factor (LIF) (AA 23-202) protein (His-GST)LIF Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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STABILITY: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Isoelectric Point: 8.9
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute in 10 mM PBS ( pH 7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.
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Preservative
- Dithiothreitol (DTT), ProClin
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Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
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- LIF (Leukemia Inhibitory Factor (LIF))
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Alternative Name
- Leukemia Inhibitory Factor
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Background
- CDF, D-FACTOR, HILDA, MLPLI, Cholinergic Differentiation Factor, Differentiation-Stimulating Factor, Melanoma-Derived LPL Inhibitor, Emfilermin
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Molecular Weight
- 25kDa
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UniProt
- P17777
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Pathways
- JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding
Target
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