ASAH2 Protein (AA 99-780)

Catalog No. ABIN8128806

This Recombinant ASAH2 protein is produced in HEK-293F Cells.

Quick Overview for ASAH2 Protein (AA 99-780) (ABIN8128806)

Target: ASAH2 (N-Acylsphingosine Amidohydrolase (Non-Lysosomal Ceramidase) 2 (ASAH2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293F Cells

Application: Activity Assay (AcA), Cell Culture (CC)

Purity: > 80 %

Product Details

Protein Characteristics: AA 99-780

Purpose: Active N-Acylsphingosine Amidohydrolase 2 (ASAH2)

Sequence: Phe99-Ile780

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

STABILITY: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Isoelectric Point: 7.2

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 20 mM Tris, 150 mM NaCl ( pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Target Details

Target: ASAH2 (N-Acylsphingosine Amidohydrolase (Non-Lysosomal Ceramidase) 2 (ASAH2))

Alternative Name: N-Acylsphingosine Amidohydrolase 2

Background: HNAC1, non-lysosomal ceramidase, Neutral ceramidase

Molecular Weight: 100kDa

UniProt: Q9NR71