SPARC Protein (AA 18-303)
Quick Overview for SPARC Protein (AA 18-303) (ABIN8129212)
Target
See all SPARC ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 18-303
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Purpose
- Active Osteonectin (ON)
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Sequence
- Ala18-Ile303
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Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303) (Active) protein (His tag)
SPARC Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 99-330) protein (Fc Tag)SPARC Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-297) protein (His-IF2DI Tag)SPARC Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 1-303) protein (GST tag)SPARC Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-302) protein (His tag)SPARC Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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STABILITY: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Isoelectric Point: 4.4
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute in 10 mM PBS (PH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.
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Preservative
- Dithiothreitol (DTT), ProClin
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Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
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- SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))
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Alternative Name
- Osteonectin
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Background
- SPARC, BM-40, Secreted Protein,Acidic,Cysteine-Rich, Basement-membrane protein 40, Secreted protein acidic and rich in cysteine
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Molecular Weight
- 44kDa
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UniProt
- P09486
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Pathways
- Autophagy
Target
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