Caspase 9 Protein

Catalog No. ABIN925032

This Recombinant Caspase 9 protein is expressed in Escherichia coli (E. coli).

Quick Overview for Caspase 9 Protein (ABIN925032)

Target: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: 90 % by SDS-PAGE

Product Details

Characteristics: 400 units/mg. One unit is the enzyme activity that cleaves 1 nmol of the caspase substrate LEHD-pNA per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -80 °C

Target Details

Target: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Alternative Name: Caspase-9

Background: Active enzyme was expressed in E. coli. The expressed caspase-9 spontaneously undergoes autoprocessing to yield the subunits characteristic of the native enzyme. One unit of rhCaspase-9 is the enzyme activity that cleaves 1 nmol of substrate Ac-LEHD-pNA per hour at 37°C at standard substrate concentrations. In combination with caspase-9 activity assays, the active rhCaspase-9 is useful in screening caspase inhibitors for pharmaceutical applications. The enzyme is also useful in studying enzyme regulation, determining target substrates and as a positive control in caspase-9 assays.

Pathways: MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Endopeptidase Activity