IGF1 Protein

Catalog No. ABIN934623

This Recombinant IGF1 protein is expressed in Escherichia coli (E. coli).

Quick Overview for IGF1 Protein (ABIN934623)

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 98 % pure

Product Details

Sequence: GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA

Characteristics: Purified recombinant Human IGF1 protein
Expression System: E.coli
Bioactivity: The ED50 was determined by a cell proliferation assay using FDC-P1 cells is ? 2.0 ng/mL, corresponding to a specific activity of ? 5 x 10^5 units/mg.

Endotoxin Level: < 0.1 ng per µg (1 EU/µg).

Application Details

Application Notes: Each Investigator should determine their own optimal working dilution for specific applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in laboratory grade water to a concentration of 0.1 - 1.0 mg/mL.

Buffer: Lyophilized with no additives. DO NOT VORTEX.

Preservative: Without preservative

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.

Target Details

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Alternative Name: IGF1

Background: The IGFs are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. IGFs are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the Insulin gene family, which also contains insulin and relaxin. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone. Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but, IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor.
Alternative Names: IGF 1 protein, IGF 1, IGF-1 protein, IGF1, Insulin-like Growth Factor-1 protein, IGF-1A protein, Somatamedin C protein, IGF-1

Pathways: RTK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation