TXN Protein

Catalog No. ABIN934926

This Recombinant TXN protein is expressed in Escherichia coli (E. coli).

Quick Overview for TXN Protein (ABIN934926)

Target: TXN (Thioredoxin (TXN))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 % pure

Product Details

Sequence: MVKQIESKTA FQEALDAAGD KLVVVDFSAT WCGPCKMIKP FFHSLSEKYS NVIFLEVDVD DCQDVASECE VKCMPTFQFF KKGQKVGEFS GANKEKLEAT INELV

Characteristics: Purified recombinant Human Thioredoxin1 protein
Expression System: E.coli
Bioactivity: Specific activity is 7-10 A650/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.
Molecular weight on SDS-PAGE will appear higher.

Application Details

Application Notes: Thioredoxin1 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Assay Procedure:

1. Prepare a 1 mL reaction cocktail in a suitable container. The final concentrations are 1 mM Tris, 61 mM sodium phosphate, 1 mM DTT, 0.1 % (w/v) insulin, 1 mM EDTA.
   2. Add 4 µg of recombinant thioredoxin into reaction cocktail and mix immediately.
   3. Incubate at 25 °C for 20 minutes.
   4. Record the increase in A650 nm for 15 minutes

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Supplied as a liquid in PBS, pH 7.4.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: RT/-20 °C

Storage Comment: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

Target Details

Target: TXN (Thioredoxin (TXN))

Alternative Name: Thioredoxin1

Background: Thioredoxin (Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. It is involved in the first unique step in DNA synthesis. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation. Recombinant human thioredoxin-1 was overexpressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names: ATL derived factor protein, TXN protein protein, Thioredoxin 1 protein, TR-I protein, TRX1 protein, TXN protein, TRX protein, , DKFZp686B1993 protein, Thioredoxin1, Surface associated sulphydryl protein protein, Thioredoxin protein, Thioredoxin -1 protein, Thioredoxin I protein, TRX 1 protein, Thioredoxin-2 protein, Thioredoxin-1 protein, ADF protein, SASP protein, Thioredoxin 1, TRDX protein, TRX2 protein, MGC61975 protein, Thioredoxin -1, Thioredoxin-1 protein

Molecular Weight: 11.7 kDa (105 AA)

Pathways: Carbohydrate Homeostasis, Cell RedoxHomeostasis