IL-2 Protein (C145S)

Catalog No. ABIN934929

This Recombinant IL-2 protein is expressed in Escherichia coli (E. coli).

Quick Overview for IL-2 Protein (C145S) (ABIN934929)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 % pure

Product Details

Protein Characteristics: C145S

Sequence: MPTSSSTKKT QLQLEHLLLD LQMILNGINN YKNPKLTRML TFKFYMPKKA TELKHLQCLE EELKPLEEVL NLAQSKNFHL RPRDLISNIN VIVLELKGSE TTFMCEYADE TATIVEFLNR WITFSQSIIS TLT

Characteristics: Purified recombinant Human IL2 protein
Expression System: E.coli
Bioactivity: Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 for this effect is < 1.5 ng/mL.
Molecular weight on SDS-PAGE will appear higher.

Application Details

Application Notes: IL2 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Assay Procedure:

Cell line: CTLL2 (mouse cytotoxic T-cell) Maintenance Condition: 10 % FBS RPMI 1640 with hIL2 Assay medium: 10 % FBS RPMI 1640 without hIL2 Cell Density: 2 x 10,000 cells/well (96 well plate, final volume 100 µL) Starvation: 24 h, Assay medium Incubation Time: 24 h (after sample treatment) Concentration Range: 0.039 ng/mL - 40 ng/mL Detection method: MTT assay

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Supplied as a liquid in PBS, pH 7.4.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: RT/-20 °C

Storage Comment: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

Target Details

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: IL2

Background: IL-2, also known as T cell growth factor(TCGF), is an immunoregulatory lymphokine produced by T-cells in response to protein or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes. This recombinant human IL-2 is a 15.4 kDa protein, containing 133 amino acid residues including one intra chain disulfide bond. Recombinant human IL-2 was overexpressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Alternative Names: T-cell growth factor protein, Interleukin 2 protein, IL-2, IL-2 protein, IL 2 protein, IL-2 protein, Aldesleukin protein, Interleukin-2 protein, IL 2, IL2, IL 2 protein, TCGF protein

Molecular Weight: 15.4 kDa (133 AA)

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation